Can High-Speed ​​Countercurrent Chromatography Extract Xanthohumol?

Yog lawm.Hops Xanthohumol hmoov, ib tug prenylated flavonoid pom nyob rau hauv hops, yuav muab rho tawm siv high-ceev countercurrent chromatography (HSCCC). Cov compounds uas tsis tshua muaj solubility los yog cov rhiab heev rau cov cua sov los yog cov kuab tshuaj muaj peev xwm muab tau zoo sib cais thiab purified los ntawm complex mixs siv cov txheej txheem HSCCC. Xanthohumol muaj qhov hnyav molecular hnyav thiab yog soluble hauv nonpolar thiab nruab nrab polar solvents. HSCCC siv cov txheej txheem ua kua-kua faib, uas yog qhov zoo rau kev sib cais flavonoids thiab lwm yam khoom ntuj. Cov khoom sib xyaw zoo li xanthohumol tuaj yeem sib cais siv txoj kev no yam tsis muaj kev pheej hmoo ntawm degradation uas qee zaum tshwm sim nrog cov txheej txheem sib cais ntau dua li cov kab ke pa chromatography vim nws maj mam, hnyav, thiab tsis muaj kev puas tsuaj. Cov tsis tseem ceeb hauv kev rho tawm ntawm xanthohumol siv HSCCC yuav suav nrog kev xaiv cov kuab tshuaj tsim nyog uas ua kom zoo dua qhov kev faib tawm ntawm cov theem nyob ruaj ruaj thiab txawb thiab kho tus nqi ntws thiab lwm yam kev ua haujlwm kom ua tiav qhov kev sib cais zoo tshaj plaws.

 

 

High-Speed ​​Countercurrent Chromatography (HSCCC) yog ib hom kua-kua chromatography txheej txheem uas siv ob theem ua kua immiscible los cais cov tebchaw raws li lawv tus cwj pwm faib ntawm ob theem. Tsis zoo li cov khoom siv-theem chromatography ib txwm muaj, qhov chaw nyob ruaj khov feem ntau yog cov khoom zoo (xws li silica gel), HSCCC siv ob theem ua kua, nrog rau ib theem yog theem nyob ruaj khov (tseem nyob hauv ib kem) thiab lwm yam raws li theem mobile (ntws los ntawm cov system).

HSCCC feem ntau siv los cais cov sib tov sib xyaw, tshwj xeeb tshaj yog rau cov tshuaj, biomolecules, thiab cov khoom ntuj. Nws cov txiaj ntsig suav nrog kev daws teeb meem siab, cov khoom lag luam tsawg tsawg, thiab muaj peev xwm cais cov sib txuas uas cov txheej txheem chromatography cov txheej txheem yuav nyuaj.

 HSCCC ua haujlwm li cas

A. Ob theem ua kua

a. Phase nyob ruaj ruaj: Ib qho ntawm cov kua theem yog nyob ruaj ruaj (tsis txav) thiab feem ntau khaws cia rau hauv kem vim lub ntiajteb txawj nqus lossis lub zog centrifugal.

b. Mobile Phase: Qhov thib ob ua kua theem yog txawb thiab txuas ntxiv ntws los ntawm kem, tso cai rau cov khoom txav thiab cuam tshuam nrog cov theem nyob ruaj khov.

B. Txoj Cai Sib Cais (Partitioning)

a. Kev sib cais hauv HSCCC yog ua raws li lub hauv paus ntsiab lus ntawm kev faib chromatography. Cov tshuaj nyob rau hauv cov khoom muab faib nruab nrab ntawm ob theem immiscible kua. Cov tebchaw yuav faib lawv tus kheej ntawm cov theem nyob ruaj ruaj thiab mobile nyob ntawm seb lawv cov txheeb ze solubility (kev faib coefficient) hauv txhua theem.

b. Ib qho kev sib xyaw ua ke uas muaj ntau dua soluble hauv lub sijhawm txawb yuav txav nrawm dua los ntawm kem, thaum lub compound ntau soluble nyob rau hauv qhov chaw nres tsheb yuav cuam tshuam ntau dua thiab mus qeeb dua.

C. Centrifugal Force

a. Ib qho ntawm cov yam ntxwv tseem ceeb ntawm HSCCC yog kev siv lub zog centrifugal (hauv HSCCC ntsuas) txhawm rau ua kom cov kua dej tsis sib haum los ntawm kev sib xyaw. Qhov no feem ntau yog ua tiav los ntawm kev tig kab ntawm kev kub ceev, uas tsim cov centrifugal rog uas pab tswj cov kua-kua theem system.

b. Qhov kev quab yuam centrifugal no tseem ua lub luag haujlwm tseem ceeb hauv kev yuam cov theem ntawm lub xov tooj ntawm tes los ntawm cov theem nyob ruaj khov, tso cai rau kev sib cais ntawm cov tebchaw raws li lawv qhov sib faib coefficients.

D. Gradient Elution

a. Ib yam li nyob rau hauv ib txwm chromatography, HSCCC tuaj yeem ua haujlwm gradient elution, qhov twg muaj pes tsawg leeg ntawm lub xov tooj ntawm tes hloov lub sijhawm. Qhov no yog qhov muaj txiaj ntsig zoo thaum cuam tshuam nrog cov khoom lag luam uas muaj ntau yam ntawm cov polarities, raws li cov theem ntawm cov khoom siv mobile tuaj yeem hloov kho kom zoo rau kev sib cais.

E. Kev kuaj xyuas

a. Tom qab kev sib cais tiav lawm, cov khoom sib txawv tuaj yeem raug sau los ntawm cov chaw sib txawv ntawm HSCCC system. Ntau yam khoom siv tuaj yeem siv los saib xyuas cov khoom sib cais, xws li UV-Vis absorbance detectors, fluorescence detectors, lossis mass spectrometry.

 Cov ntsiab lus tseem ceeb ntawm HSCCC

A. HSCCC Instrument:

Qhov no feem ntau muaj xws li:

a. Rotating Kem: Ib sab cylindrical uas yog spun ntawm kev kub ceev. Nws yog sau nrog ib qho ntawm cov kua theem immiscible.

b. Liquid Reservoirs: Cov no tuav ob lub sijhawm ua kua tsis zoo uas tau muab tso rau hauv qhov system.

c. Product Injection System: Qhov no yog qhov uas cov khoom tau nkag mus rau hauv chromatography system.

d. Elution System: Lub kaw lus uas tso cov theem ntawm lub xov tooj ntawm tes los ntawm cov kab rotating.

e. Fraction Collector: Qhov no sau cov khoom sib cais tom qab lawv tawm hauv kem.

B. Phase System: Ob theem ua kua immiscible siv hauv HSCCC yuav tsum tau ua tib zoo xaiv. Cov no tuaj yeem suav nrog:

a. Aqueous-organic mixtures: Feem ntau siv cov kuab tshuaj xws li dej-organic hnyav tov, piv txwv li, dej-ethyl acetate.

b. Ob cov kuab tshuaj organic: Hauv qee kis, ob qho organic solvents tuaj yeem siv tau.

 Qhov zoo ntawm HSCCC

A. Tsis Siv Neeg Nyob ruaj ruaj

HSCCC tsis tso siab rau cov theem ruaj khov zoo li silica gel, uas txhais tau hais tias tsis muaj kev pheej hmoo ntawm kev hloov tsis tau adsorption lossis khoom degradation vim muaj kev cuam tshuam nrog cov theem nyob ruaj khov.

B. Kev daws teeb meem siab

Kev sib cais efficiency feem ntau yog siab, uas yog tshwj xeeb tshaj yog zoo rau purifying tej yam ntuj tso khoom los yog lwm yam complex mixs uas lub Cheebtsam muaj zoo heev zog.

C. Tsis muaj kev puas tsuaj

Raws li HSCCC cuam tshuam cov kua-kua sib cuam tshuam, nws feem ntau ua rau cov txheej txheem sib cais yooj yim dua, txo qis kev degradation lossis poob ntawm cov khoom sib txuas.

D. Yam khoom poob tsawg heev

Txij li cov txheej txheem siv cov theem ua kua nkaus xwb, cov khoom poob feem ntau qis dua piv rau cov txheej txheem chromatographic.

E. Ntau yam

Nws tuaj yeem siv rau ntau yam tshuaj, suav nrog tshuaj, biomolecules, thiab cov khoom ntuj tsim nrog ntau yam polarities.

 Daim ntawv thov

HSCCC tau siv dav hauv kev lag luam tshuaj, chemistry, biotechnology, thiab kev cais cov khoom ntuj. Cov ntawv thov tshwj xeeb suav nrog:

a. Purification ntawm Natural Products: Rau cais cov bioactive compounds los ntawm cov nroj tsuag extracts, marine kab mob, los yog fermentation broths.

b. Kev rho tawm ntawm Pharmaceutical Compounds: Rau kev sib cais thiab ntxuav cov tshuaj molecules los ntawm kev sib xyaw ua ke.

c. Purification ntawm Biomolecules: Xws li cov proteins, nucleic acids, thiab peptides.

d. Enantioseparation: Txhawm rau cais cov chiral compounds, uas yog ib qho tseem ceeb hauv kev lag luam kws tshuaj los tsim cov tshuaj ntshiab optically.

 

 

Ob peb cov nroj tsuag rho tawm tau ua tiav tiav siv HSCCC los cais ntau hom bioactive compounds. Hauv qab no yog qee cov khoom cog qoob loo uas tau muab cais los ntawm txoj kev no:

★ Flavonoids

a. Rutin los ntawm Ruta graveolens (Rue)

b. Quercetin los ntawm Hibiscus rosa-sinensis thiab Allium cepa (dos)

c. Luteolin los ntawm Chamomilla recutita (chamomile)

★ Alkaloids

a. Berberine los ntawm Berberis vulgaris (Barberry)

b. Sparteine ​​los ntawm Cytisus scoparius (Scotch broom)

c. Corydine los ntawm Corydalis hom

★ Terpenoids thiab Essential Oils

a. Caryophyllene los ntawm Cinnamomum verum (Cinnamon)

b. Artemisinin los ntawm Artemisia annua (Sweet Wormwood)

c. Triterpenes los ntawm Ganoderma lucidum (Reishi Mushroom)

d. Limonene los ntawm Citrus sinensis (txiv kab ntxwv tev)

★ Phenolic Acids

a. Caffeic acid los ntawm Echinacea purpurea

b. Chlorogenic acid los ntawm Coffea arabica (Coffee)

c. Gallic acid los ntawm Camellia sinensis (Tea)

d. Rosmarinic acid los ntawm Rosmarinus officinalis (Rosemary)

★ Glycosides

a. Anthraquinone glycosides los ntawm Rheum palmatum (Rhubarb)

b. Saponins los ntawm Glycyrrhiza glabra (Licorice)

c. Ginsenosides los ntawm Panax ginseng (Ginseng)

d. Geniposide los ntawm Gardenia jasminoides

★ Flavonoid Glycosides

a. Isoquercitrin los ntawm Cucumis sativus (Cucumber)

b. Hyperoside los ntawm Hypericum perforatum (St. John's Wort)

★ Coumarins

a. Scopolia los ntawm Scopolia japonica

b. Umbelliferone los ntawm Coriandrum sativum (Coriander)

★ Steroids

a. -Sitosterol los ntawm Pygeum africanum (African plum ntoo)

b. Stigmasterol los ntawm Glycine max (Soybean)

c. Campesterol los ntawm Brassica oleracea (Cabbage)

★ Polysaccharides

a. Ganoderan los ntawm Ganoderma lucidum (Reishi Mushroom)

b. Chitosan los ntawm Mushrooms (tshwj xeeb yog Ciniella hom)

★ Amino Acids thiab Peptides

a. Aminopeptidase inhibitors los ntawm Pisum sativum (Peas)

b. Glutathione los ntawm Spinacia oleracea (Spinach)

 

Inhealth Nature qhov kev cog lus rau ntau tshaj kaum xyoo hauv cov khoom xyaw R & D ci los ntawm nws cov khoom hwm,Hops Xanthohumol hmoov. Cov khoom no, meticulously tsim thiab tsim, embodies Inhealth Nature txoj kev koom tes nrog zoo thiab ua tau zoo. Cov lus pom zoo rau cov neeg siv khoom txaus siab yog ib qho pov thawj ntawm Xanthohumol qhov ua tau zoo heev. Los ntawm kev xaiv Inhealth Nature's Xanthohumol, koj tab tom xaiv tus khub uas muaj kev ntseeg siab nrog cov ntaub ntawv ua tau zoo, tsis yog cov khoom nyoos xwb.

Txais tos tiv tauj peb ntawmbella@inhealthnature.comyog tias koj muaj lus nug lossis lus nug.